The Asskicker Room is a location on the Shores of Acheron. Here Dante finds Lucifer with Beatrice, who taunts him about his lost love. After Lucifer flies away, several Guardian Demons and Minions spawn, and a battle ensues. This room is just before the rope to board Charon.
- The name of this room is derivitive of the concept art for Dante's Inferno, in which the art for was titled "Asskicker" Room.
- This room is the one shown in the upgrade menu, where the player can spend souls.